TABLE 1.

Conventional PCR primers for the detection of RhCMV^a

Primer pair	Primer sequence (5′-3′) and location				Expected amplicon size
	Forward primer	Location	Reverse primer	Location	RhCMV-WT	RhCMV 180.92
IE1	TCAGGGTGGCATGGAACTCAT	UL123	TGCACACTCCCATTACCATGC	UL123	171	171
UL146	TCAAACACCGACGGGTTACG	UL146	AGGGTTCGGAACTACGTTGCAG	UL146	172	Absent
ΔCED	GCACAGTAGATGATGATACCCAAGAG	UL132	GGCTTTCTGCATATCAGTGCTTTGGACGGG	Upstream rh167	9,179	912
ULb′-1	GCACAGTAGATGATGATACCCAAGAG	UL132	GCACCATTCCAAATGGTAACGG	rh161.2	3,875	Absent
ULb′-2	GCACAGTAGATGATGATACCCAAGAG	UL132	GCAGCGTACTTCGTGTGCTGTACCGAAATC	UL146	1,977	Absent
ULb′-3	GTGCGATGTACACTCGCAGGAAGTCT	Upstream UL146b	CTACATATGGCAAGATGCTCACGGCCTACATGTAC	UL144	1,868	Absent
ULb′-4	GGCTTTCTGCATATCAGTGCTTTGGACGGG	Upstream UL145	CACCATCGTAATGGTGAGCCTGAC	UL141	1,859	Absent

^aBased on GenBank accession numbers EF990255 for RhCMV-WT and DQ120516 for RhCMV 180.92.