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Oligomeric state of recombinant protein V. (A) SDS-PAGE analyses of recombinant protein V (5 μg) after cross-linking with different amounts of DMP. Samples were analyzed on a 4 to 20% SDS-PAGE and stained with Simply Blue dye. The locations of the monomer (M) and dimer (D) are indicated. (B) Chromatogram of purified protein V subjected to SEC-MALS showing the average molecular weight (Mw; in thousands) of the elution peak and the polydispersity values. LS, light scatter.
