TABLE 1.
Quantification of protein V purity and yield
| Sample sourcea | Total protein concn (mg/liter of culture) | Purity (%) | Protein V (mg) | A260/280 ratiob |
|---|---|---|---|---|
| Total | 1,851.5 | 12.7 | 235.3 | 1.85 |
| Supernatant | 1,382.5 | 15.1 | 209.3 | 1.4 |
| ElutionStrep | 14.1 | 94.4 | 13.3 | 0.8 |
| ElutionNi | 8.8 | 99.0 | 8.7 | 0.7 |
| Protein V | 4.7 | 99.1 | 4.7 | 0.7 |
a
Supernatant, supernatant after PEI precipitation; ElutionStrep, elution with Strep-tag; ElutionNi, elution with Ni-NTA; Protein V, protein V without tags after SEC.
b
Ratio of absorbance at 260 and 280 nm.