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. 2014 Aug;88(16):9297–9309. doi: 10.1128/JVI.00990-14

FIG 1.

FIG 1

Schematic of CyHV-3 genome and locations of primers and DNA probe. (A) CyHV-3 genomic structure. The viral terminal repeats (TR) (containing ORF1 to ORF8) and unique regions (UR) containing ORF9 to ORF156 are indicated. (B) Expanded TR with ORF1 to -8; notched arrows indicate introns for ORF6 and ORF7. (C) Expanded 5′-3′ ORF6. The dashed line indicates the 86-bp intron. (D) Relative locations of the primers used in 5′ RACE and 3′ RACE and primers specific for the ORF6 flanking the intron. (E) Relative locations of the DNA probe specific for the spliced and unspliced ORF6. The expected sizes of the PCR products for the spliced ORF6 are indicated above the bars for primers ORF6s-F291 and -R291 or primers ORF6s-F216 and -R216.