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. 2014 Aug;88(16):9297–9309. doi: 10.1128/JVI.00990-14

FIG 4.

FIG 4

Real-time PCR of koi WBC DNA. (A) Standard curve using threshold cycles to calculate the analytic sensitivity of CyHV-3 genome equivalents with the real-time TaqMan PCR. (B) The white bars show the copy number of CyHV-3 genomes detected from 1 × 106 cells of selected (IgM+ B cells) and nonselected (IgM−) WBC from CyHV-3 latently infected koi. The mean genome copy numbers were 3,917.0 ± 496.0 for IgM+ cells and 191.9 ± 32.4 for IgM− cells (n = 3) (P = 0.0017 by two-tailed t test). The cycle threshold (CT) values for the Cyprinus carpio glucokinase housekeeping gene from 1 × 106 cells from selected (IgM+ B cells) and nonselected (IgM−) WBC from CyHV-3 latently infected koi. The mean CT values of the Cyprinus carpio glucokinase housekeeping gene were 19.55 ± 0.055 for IgM+ cells and 19.93 ± 2.78 for IgM− cells (n = 2) (P = 0.9026 by two-tailed t test).