Skip to main content
. 2014 Aug;88(16):9297–9309. doi: 10.1128/JVI.00990-14

FIG 5.

FIG 5

Autoradiogram of CyHV-3 ORF6 RT-PCR amplicons. cDNA was synthesized with random primer from total RNA of leukocytes isolated from CyHV-3 latently infected koi: sorted IgM+ WBC (B cells) (lanes 1 and 2), IgM WBC (lanes 3 and 4) and KF-1 infected cells (lanes 6 and 7). Lane 5 is the positive control and contains CyHV-3 KHV-U DNA. Lane 8 is the negative control without nucleic acid). The presence (+) or absence (−) of reverse transcriptase (RT) in the cDNA synthesis reaction is shown. The PCR primers are ORF6s-F291 and ORF6s-R291. The membrane was probed with DIG-labeled DNA probe described in the legend to Fig. 1E, which is specific for ORF6 and internal to RT-PCR primers. The leftmost lane (lane MS) contains DNA Molecular Weight Marker VII (DIG labeled) (Roche Applied Science). The positions of the molecular size markers (in base pairs) estimated by using the molecular weight markers are indicated at the sides of the gel.