TABLE 1.
Primer pairs used in RT-PCR, PCR, and RACE
| Assay | Primera | KHV target | Primer sequence (5′ to 3′) |
|---|---|---|---|
| RT-PCR | ORF1-F340 | ORF1 | TGAGCACAGGACTGCTGATT |
| ORF1-R430 | AGGGACCAGGGTCTTCATCT | ||
| ORF2-F377 | ORF2 | ATCATGGTCTGGCTGAGGAC | |
| ORF2-R377 | GAGATATCCCCTGCCACTGA | ||
| ORF3-F433 | ORF3 | AGCTGCTGAGAAAGCTGAGG | |
| ORF3-R433 | CCAGGTGCAGAGTTGTCAGA | ||
| ORF4-F399 | ORF4 | TGGTTCCTATGGCTGGGTAG | |
| ORF4-R399 | CCGTCTCTGGGATGAGTGTT | ||
| ORF5-F365 | ORF5 | TCAGCAGGACCAGGACTCTT | |
| ORF5-R365 | ACAGCTCGTCGTACACGTTG | ||
| ORF6s-F291 | ORF6 | GACCCAGGGGACAGCTCTAT | |
| ORF6s-R291 | AGTGGTACAAGTGGCGCTTC | ||
| ORF7s-F316 | ORF7 | CATCACTCAGCTGTGCCACT | |
| ORF7s-R316 | CGCAAGAGAGCAGTGATGAA | ||
| ORF8-F360 | ORF8 | TAAGACGTCGTGCTGTCAGG | |
| ORF8-R360 | CAGGCTGAAGTGTGAGGTGA | ||
| Southern blotting | ORF6s-F216 | ORF6 | CAGCAGACTGAGACGCTGAA |
| ORF6s-R216 | TGCACCATGGACAGACAGAT | ||
| RACE | ORF6-3RACE112 | ORF6 | AAGAAGCACAGGAGCGAGAG |
| ORF6-3RACE94 | AGGGAGAGGAGCGAGAAGAG | ||
| ORF6-5RACE599 | GTGAACTGCACCCAGTCAAA | ||
| ORF6-5RACE455 | GTGCCCAGCTCAAACTTCTC | ||
| ORF6-5RACE293 | GCAACAGCGTGTCTCTGGTA |
a
The letter after the hyphen indicates whether a primer is forward (F) or reverse (R).