FIG 11.

In ORF50-expressing cells, TLR2 and TLR4 no longer localize to the plasma membrane. HEK293T cells were cotransfected with the indicated plasmids and images were obtained 24 h posttransfection in live cells by confocal microscopy. Cherry-KDEL (red) was used as a marker for cotransfection and the endoplasmic reticulum and included in all transfections. Cells were cotransfected with TLR2-GFP and either empty pcDNA vector, K4.2, ORF21, ORF31, ORF50, or K10.5 (A) or with combinations of TLR4-YFP, TLR2-YFP, or CD95-YFP and empty pcDNA vector or ORF50 (B). Scale bar, 10 μm.