FIG 2.

MHV68-infected primary BMDMs do not respond to TLR stimulation. At 24 h postinfection, MHV68- or MHV68-GFP-infected (MOI of 2) or uninfected primary BMDMs were stimulated for 4 h with TLR agonists (TLR2, FSL-1; TLR9, CpG B) in the presence of brefeldin A to prevent TNF-α secretion and then processed for intracellular MHV68 and TNF-α staining and analyzed by flow cytometry. (A) Representative contour plots. (B) The averages + SD of the mean fluorescence intensity of the anti-TNF-α channel gated on MHV68-negative (uninfected) or MHV68-positive (MHV68 or MHV68-GFP) populations from 2 independent experiments are shown. *, P < 0.05.