Skip to main content
. 2014 Aug;88(16):8843–8852. doi: 10.1128/JVI.00424-14

FIG 5.

FIG 5

IAV NS1 expression induces JNK activation. (A) pHW2000- and pMPccdB-based plasmids encoding the eight genomic segments of A/FPV/Bratislava/79 were transfected into A549 cells. Additionally, the pHW2000 plasmids containing NS segments of A/Puerto Rico/8/34 (H1N1) (PR8), A/Hamburg/4/09 (H1N1) (Hamburg), A/Thailand/1(Kan-1)/04 (H5N1) (Thailand), and A/Vietnam/1203/04 (H5N1) (Vietnam) were transfected into cells. After 24 h, cells were harvested and analyzed by Western blotting. M, matrix protein; NP, nuclear protein; HA, hemagglutinin; PB2, polymerase B2; NA, neuraminidase; PB1, polymerase B1; PA, polymerase A. (B) pcDNA3 plasmids expressing NS1s from IAVs of various subtypes were transfected into cells, and phosphorylation of JNK was analyzed by Western blotting. In the cases of the Guangdong and Rostock viruses, either the monoclonal NS1 antibody could not detect the subtype-specific NS1 protein or expression was too low to be detected. Guangd., NS1 from A/Goose/Guangdong/3/97 (H5N1); Rostock, A/FPV/Rostock/34 (H7N1); WSN, A/WSN/1933 (H1N1); PR8, A/Puerto Rico/8/34 (H1N1); FluB, influenza B virus; PR8Mt.Si, A/Puerto Rico/8/34/Mount Sinai (H1N1); Bratislava, NS1 from FPV/Bratislava/79 (H7N7); M1 Bratislava, M1 from FPV/Bratislava/79 (H7N7); SC35M, SC35M (H7N7).