TABLE 1.

Comparison of the amino acid sequences ofthe NS1 proteins of JNK-activating and nonactivating IAV subtypes^a

^aAlignment of amino acid sequences of NS1s of two JNK-activating viruses, IAV SC35M (H7/N7) (SC35M) and A/FPV/Rostock/34 (H7N1) (Rostock), and two nonactivating viruses, A/Puerto Rico/8/34 (H1N1) (PR8) and A/WSN/1933 (H1N1) (WSN). Positions that have been mutagenized are marked. Seven amino acids (positions 3, 22, 81, 114, 215, 225, and 228) were initially identified but were experimentally disproved as being involved in JNK activation. Below the amino acid sequences, the amino acids around position 103 of the NS1 proteins of all IAVs used in this study are shown. Wisco, Wisconsin.