FIG 2.
Overexpression of Sti1p inhibits CIRV accumulation in yeast. (A) Top panel, Northern blot analysis of CIRV RNA accumulation in wt or sti1Δ yeasts overproducing the FLAG-tagged Sti1p. Second panel, Northern blot analysis to demonstrate the comparable level of rRNA loading in the yeast samples. Bottom panels, Western blot analysis of CIRV His6-p95 and CIRV His6-p36 accumulation by anti-His antibody and Sti1p accumulation by anti-Sti1 antibody. (B) Northern blot analysis of TBSV DI-72 repRNA accumulation in wt or sti1Δ yeasts overproducing the FLAG-tagged Sti1p in the presence of peroxisomal CNV p33/p92 replication proteins. Each experiment was repeated three times. See further details in legend to panel A.