FIG 6.

Functional TPR1 domain of Sti1p is required for inhibition of CIRV replication in yeast. (A) Northern blot analysis of CIRV accumulation in wt yeast overproducing the FLAG-tagged Sti1p or derivatives. Bottom panel, Northern blot analysis to demonstrate the comparable level of rRNA loading in the yeast samples. (B) Western blot analysis of CIRV His₆-p36, and CIRV His₆-p95 accumulation by anti-His antibody from yeast overproducing Sti1p or derivatives. (C) Detection of the overproduced FLAG-Sti1p or its derivatives in yeast by Western blotting using anti-FLAG antibody. Each experiment was repeated three times.