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Characterization of VP1 protein expression by rNDVs in DF1 cells and embryonated eggs. (A) Kinetic analysis of VP1 expression by rNDVs in DF1 cells. Cell lysates and culture medium were collected every 12 h and subjected to Western blot analysis. (B) Detection of norovirus VP1 in allantoic fluid of embryonated chicken eggs. Allantoic fluids were harvested at 72 h postinfection and clarified by centrifugation at 3,000 rpm for 10 min.
