FIG 3.

Incorporation of chimera proteins into VLPs. Avian cells were transfected with empty vector or combinations of cDNAs encoding the genes indicated at the bottom. Equivalent amounts of DNA were used for all cDNAs. VLPs, harvested from 48 to 96 h after transfection, were purified as described in Materials and Methods, and proteins present in the purified particles were separated on SDS-polyacrylamide gels in the presence of reducing agent. The F-protein chimera proteins F/F and F/HR2F were detected with anti-RSV F-protein HR2 peptide antibody, while the H/G chimera protein was detected with anti-RSV antibody, which detects only G protein and not F protein on Western blots (26), in contrast to F protein detection by this antibody by immunofluorescence, as in Fig. 2.