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Antigenic site ϕ in chimera proteins. (A) Cells transfected with pCAGGS-F/F, pCAGGS-F/HR2F, or the pCAGGS-F wild type (wt) for 48 h were analyzed by flow cytometry for binding to anti-5C4 and to anti-1112 MAbs, as described in Materials and Methods. The graph shows the ratios of the mean intensity of fluorescence for binding of the two antibodies, 5C4 and 1112. (B and C) VLP-HG+F/F and VLP-H/G+F/HR2F binding to increasing amounts of MAb 1112 (B) and MAb 5C4 (C).
