FIG 7.

Transmission of SsPV1/WF-1 between vegetatively incompatible host strains. (A) Colony morphology of strains R6, R6V, and 1980/R6V. R6V represents the strain that arises from R6 after contact with hypovirulent strain WF-1, whereas 1980/R6V represents strain 1980 after dual culturing and contact with strain R6V. (B) Colony morphology of strains SM-1, SM-1V, and 1980/SM-1V. SM-1V is a strain of S. minor SM-1 after strain SM-1 was dually cultured with strain WF-1 on a PDAY plate. 1980/SM-1V was a subculture that was cut out from side of strain 1980 after mycelia of strain SM-1V and 1980 contacted on a PDAY plate. (C) dsRNA extraction and RT-PCR detection of SsPV1/WF-1 in individual strains. All dsRNA samples were treated with DNase I and S1 nuclease prior to electrophoresis. The actin gene was used as an internal control. The predicted lengths of the RT-PCR products for CP and RdRp of SsPV1/WF-1 are 300 and 320 nt, whereas the predicted PCR products from SsHV1/SZ150, SsRV/SZ150, and SmRV/SM-1 are 252, 462, and 424 nt, respectively. The primers for virus detection are shown in Table S1 in the supplemental material.