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Virus-shedding titers of SPF chickens inoculated with SH7, SH14, and mutated viruses. The SPF chickens were inoculated with 106 EID50 SH7, SH14, 7HAK363R, and 7PAL672F. Oropharyngeal swabs and cloacal swabs were collected 3, 5, 7, and 9 dpi. Viral titers of swabs were determined by endpoint titration in SPF embryonated eggs. Each pattern bar represents the viral titers from an individual chicken. The dashed horizontal line indicates the lower limit of detection.
