FIG 2.

Comparative epitope specificities and magnitudes of responses to VACV- and ECTV-primed splenocytes. Naive splenocytes were incubated with synthetic poxvirus peptides ranging from 12 to 15 amino acids in length and screened for reactivity against splenocytes from ECTV-primed mice 10 days postinfection. Naive splenocytes were incubated with peptide (final concentration, 2 μg/ml). Peptide-primed splenocytes were coincubated overnight with whole splenocytes from ECTV-primed mice (3,000 PFU per footpad [f.p.]) 10 days postinfection. IFN-γ-positive T cell responses were assayed by IFN-γ ELISpot. To determine the level of statistical significance, Student's t test was performed using the mean of triplicate values of the response. #, number; *, P < 0.05; limits, means ± standard deviations (SD); APC, antigen-presenting cell. Data are representative of 3 independent experiments.