FIG 4.
Comparative epitope reactivities to D8L238–252 between VACV- and ECTV-primed splenocytes. Naive splenocytes were incubated with synthetic D8L238–252 (VACV sequence) or D8L238–252A244→V244/A249→V249 (ECTV sequence) at a final concentration of 2 μg/ml. Peptide-primed splenocytes were then coincubated overnight with whole splenocytes from ECTV-primed mice or VACV-primed mice (3,000 PFU per footpad) 10 days postinfection. IFN-γ-positive T cell responses were assayed by IFN-γ ELISpot. To determine the level of statistical significance, Student's t test was performed using the mean of triplicate values of the response. *, P < 0.05.