TABLE 1.

Primers used for construction of the gH-2 deletion virus

Region amplified	Primer name	Sequence (5′ to 3′)a
Region homologous to left of UL22	LL-Van91I-UL22	TTTTTTTT CCAT AAA TTGG GTT CGT GCA AGT GAA GCA CAT CG
	LR-Van91I-UL22	TTTTTTTT CCAT TTC TTGG CGC GAA TAA ACG GGT GTG
Region homologous to right of UL22	RL-DraIII-UL22	TTTTTTTT CAC AGA GTG GTC GTC CCG GCT GCC AGT C
	RR-DraIII-UL22	TTTTTTTT CAC CTT GTG CGT TCG TGG CCC TCA TGC C
Left to UL22	gH2-L-check	AAA TCA TGG GTG GAT GTG GTT CGC GAG C
	gH2-WT-Left	ATC ACC CAC AAC GCC AGC TAC G
	sacB-Out	AGGATACAGGACCTGCCAAT
Right to UL22	gH2-R-check	GGT GAG GAT GCT TGG CCA GAA GCG G
	gH2-WT-R-Out	GGC TGT CCC GCA TCG ATA TCA CG
	Hyg-Out	CTTCACCGATCCGGAGGAAC

^aUnderlining indicates Van91I and DraIII sites.