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HSV-1 DNA stimulates RPA phosphorylation in transfected cells but not infected cells. Vero cells were either infected with HSV-1 at an MOI of 10 and harvested at 3 h postinfection (KOS) or transfected with 700 ng of virion DNA and harvested 3 h following serum addition (KOS DNA). As a positive control for pRPA32 S4/S8, Vero cells were treated with 50 J/m2 and allowed to recover for 1 h at 37°C (UV).
