FIG 3.

TLR2, -3, and -4 protein expression in M1- and M2-polarized macrophages and untreated MDMs. M1- and M2-polarized macrophages and untreated MDMs were harvested and stained with MAbs against TLR2, -3, and -4. Since TLR3 is located endosomally, we performed a permeabilization step before adding the MAb. The results of one representative example of three experiments are shown. Red lines, unstained; green lines, MDMs; blue lines, M1- and M2-polarized macrophages stained with the corresponding MAb. Max, maximum; PE, phycoerythrin; BV, brilliant violet.