FIG 6.

Neutralization of IFN-α with antibodies to the IFN receptor reversed the TLR-dependent HIV-inhibitory effect only to a small extent or not at all. MDMs or TLR-primed macrophages were pretreated with neutralizing Ab (nAB) to IFN-R and subsequently infected with YU-2. HIV replication was monitored over time by quantifying the p24 in the supernatants (n = 3). The area under the curve of the p24 Ag over time is presented for each individual experiment. The agonists are described in the legend to Fig. 1. Statistical analyses were done using the paired t test with a two-tailed P value.