FIG 4.

Measured changes in the transcription rate do not match observed changes in mRNA abundance. Boxes indicate the median as well as the upper and lower quartiles, with whiskers being minimum and maximum values. Lines at y = 1 indicate no change in value (compared to that for unstimulated). The change in total mRNA abundance (green) from Gag p24-stimulated (A) and Nef-stimulated (B) CD8⁺ T cells from VCs with antiviral activity does not match the change in net transcription (blue) for MIP-1α, MIP-1αP, MIP-1β, IFN-γ, and GM-CSF. For each of these messages, increases in total mRNA for these markers were 1.5- to 3-fold more than the net transcription. In comparison, total mRNA levels from an mRNA that is known to be transcriptionally induced, TNFRSF9, increased similarly to net transcription. The same is seen for XCL-1. This indicates that other mechanisms of regulation may play a role in induction of MIP-1α, MIP-1αP, MIP-1β, IFN-γ, and GM-CSF.