FIG 6.

Virus inhibition correlates with changes in total RNA, net transcription, and apparent half-life. (A) Fold changes in p24-stimulated cells that significantly correlated with virus inhibition. (Top row) In p24-stimulated cells, the fold changes (compared to unstimulated cells) in mRNA abundances of MIP1α, MIP1α-AP, MIP1β, and XCL1 correlated with soluble virus inhibition (measured as log₁₀ reduction in virus replication), while RANTES did not. mRNA abundance was determined via primer-specific PCR. (Bottom row) Fold changes (p24 stimulated/unstimulated) in net transcription of MIP1α-AP, MIP1β, XCL1, and TNFRSF9 correlate with soluble inhibition, while RANTES again does not. The rate of transcription was determined by primer-specific PCR of 4sU-labeled RNA (described in Materials and Methods). (B) Fold changes in Nef-stimulated cells that correlate with virus inhibition. In Nef-stimulated cells, the fold change (compared to unstimulated cells) in the mRNA abundance of TNFRSF9, net transcription of IFN-γ, and apparent half-life of MIP1α correlates with soluble virus inhibition (measured as log₁₀ reduction in virus replication), while RANTES does not. The log₁₀ reduction in virus replication was determined via sVIA, as described in Materials and Methods. The r values were calculated using Spearman correlation. Trendlines are shown.