TABLE 2.
Antigen-specific CD8+ T cell inhibition of HIV-1 replication
| Patient ID | Reduction value (log10)a |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Unstimulated | Env |
Gag |
Pol |
Nef | |||||||||
| E1 | E2 | E3 | E4 | G1 | G2 | G3 | P1 | P2 | P3 | P4 | |||
| VC9 | <0.39 | <0.39 | <0.39 | 0.44 | 0.53 | 0.45 | 0.64 | <0.39 | 0.56 | 0.63 | 0.52 | 0.61 | 0.74 |
| VC11 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.40 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 |
| VC15 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.42 | <0.39 | <0.39 | 0.45 | 0.50 | <0.39 | 0.47 |
| VC16 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.44 | <0.39 | <0.39 | <0.39 | 0.42 |
| VC20 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.48 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.42 |
| VC23 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.40 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 |
| VC24 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 |
| VC26 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.43 | 0.40 | <0.39 | <0.39 | <0.39 | 0.45 |
| VC27 | <0.39 | <0.39 | <0.39 | <0.39 | 0.54 | 0.59 | 0.63 | <0.39 | 0.46 | <0.39 | 0.42 | 0.41 | 0.57 |
| VC28 | <0.39 | <0.39 | 0.47 | 0.41 | 0.42 | 0.43 | 0.42 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 |
| VC29 | <0.39 | 0.51 | 0.41 | <0.39 | <0.39 | 0.41 | 0.47 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.49 |
| VC30 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | 0.45 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 | <0.39 |
| Totalb | 0 | 1 | 2 | 2 | 3 | 4 | 9 | 1 | 4 | 2 | 3 | 2 | 7 |
The log10 reduction values for HIV-1 peptide-specific soluble inhibition by CD8+ T cells from HIV-1 VCs are shown. An sVIA was used to measure the inhibitory capacities of antigen-stimulated primary CD8+ T cells from 12 HIV-1+ virus controllers. Virus inhibition was measured as log reduction in CH040.c (transmitted/founder virus) replication without CD8+ T cell effectors. Values of >0.39-log-unit reduction in virus replication compared to a no-CD8+ T cell control indicate significant inhibition and are reported (boldface). A value of <0.39 (lightface) indicates a nonsignificant log10 reduction value. The peptide pools were divided as follows to represent HIV-1 regions based on the HXB2 amino acid number: Env1 (E1), amino acids 4 to 296 (gp120); Env2 (E2), amino acids 297 to 488 (gp120); Env3 (E3), amino acids 489 to 602 (gp41); Env4 (E4), amino acids 603 to 840 (gp41); Gag1 (G1), amino acids 1 to 128 (p17); Gag2 (G2), amino acids 131 to 361 (p24); Gag3 (G3), amino acids 362 to 486 (p17); Pol1 (P1), amino acids 1 to 152 (protease); Pol2 (P2), amino acids 156 to 447 (p51); Pol3 (P3), amino acids 452 to 709 (p51 plus p15); Pol4 (P4), amino acids 711 to 988 (p31); Nef, amino acids 1 to 193.
Total number of values of >0.39-log-unit reduction in virus replication compared to a no-CD8+ T cell control.