TABLE 3.
Intermolecularly covarying amino acid positions for DC RV proteins and codons with nonsynonymous-to-synonymous substitution rate (dN/dS) ratios of >1
| Protein | Status | Amino acid/codon position(s)a |
|---|---|---|
| VP7 | Covarying | 29, 316 |
| dN/dS > 1 | 11, 16, 19, 22, 26, 37, 38, 41, 45, 47, 48, 66, 74, 75, 87, 90, 96, 97, 101, 105, 116, 119, 120, 123, 125, 126, 146, 147, 149, 159, 178, 179, 193, 211, 212, 213, 217, 220, 221, 227, 233, 237, 238, 239, 242, 267, 268, 278, 303, 309, 316 | |
| VP4 | Covarying | 35, 78, 104, 108, 121, 125, 131, 135, 162, 173, 189, 199, 236, 254, 283, 322, 337, 338, 435, 514, 560, 577, 580, 586, 590, 593, 595, 617, 621, 689, 695, 698, 708, 713, 738, 750 |
| dN/dS > 1 | 125, 131, 144, 145, 146, 173, 189, 236, 281, 283, 303, 311, 365, 577, 586, 590, 593, 594, 595, 689, 695, 738, 739, 750 | |
| VP6 | Covarying | 55, 80, 130, 199, 252 |
| dN/dS > 1 | 54, 55, 129 | |
| VP1 | Covarying | 95, 298, 430, 555, 648, 813, 973 |
| dN/dS > 1 | 120, 156, 171, 210, 298, 285, 440, 444, 554, 555, 779, 821, 823, 859, 865, 886, 888, 893, 999, 1049, 1050 | |
| VP2 | Covarying | 27, 28, 35, 39, 62, 66, 68, 127, 136, 159, 239, 406, 416, 570, 729 |
| dN/dS > 1 | 46, 66, 68, 107, 119, 127, 128, 136, 143, 113, 228, 239, 532, 559, 570, 653, 775 | |
| VP3 | Covarying | 89, 103, 203, 214, 217, 310, 324, 363, 490, 504, 639, 762, 812, 826 |
| dN/dS > 1 | 115, 139, 200, 277, 301, 328, 346, 356, 363, 373, 452, 454, 521, 568, 681, 683, 707, 733, 735, 748, 762, 812, 814 | |
| NSP1 | Covarying | 103, 139, 146, 160, 200, 214, 216, 225, 303, 326, 385, 391, 399, 401, 422, 434, 473, 477, 485 |
| dN/dS > 1 | 90, 103, 111, 112, 119, 121, 139, 146, 163, 165, 166, 180, 188, 189, 192, 200, 230, 233, 251, 254, 265, 271, 272, 289, 301, 307, 314, 326, 338, 357, 359, 386, 389, 402, 408, 434, 436, 474, 476, 477 | |
| NSP2 | Covarying | 48, 64, 75, 91, 105, 136, 150, 175, 187, 189, 197, 200, 201, 248, 293, 307 |
| dN/dS > 1 | 24, 36, 48, 64, 75, 82, 93, 108, 136, 197, 200, 202, 248, 279, 293 | |
| NSP3 | Covarying | 9, 65, 79, 141, 155, 191, 229, 233, 235, 252, 255, 268, 278, 300, 301, 308 |
| dN/dS > 1 | 9, 65, 76, 79, 104, 229, 233, 240, 255, 257, 278, 300, 308 | |
| NSP4 | Covarying | 72 |
| dN/dS > 1 | 70, 72, 135, 161, 169 | |
| NSP5 | Covarying | 37, 41, 43, 45, 108, 121, 126, 131, 142, 188 |
| dN/dS > 1 | 45, 131, 132 | |
| NSP6 | Covarying | 18, 21, 25, 26, 54, 67, 73, 75, 76, 82, 88 |
| dN/dS > 1 | 21 |
a
The numbers are based on those of strain Wa. Positions involved in the covariance network and that have a codon dN/dS ratio of >1 are highlighted in boldface.