Fig. 5.

Repetitive deep tissue imaging through a cranial window.

A window was implanted into a female M-line mouse for imaging at depths up to 900 microns (full image stack in Fig. 3C). The images are maximum projections of images taken at approximately 770-830 microns below the pia mater. (A) Imaging session one at 105 days of age. (B) The second imaging session at 132 days of age. (C) Imaging session three at 211 days of age. (D) The final imaging session occurred at 323 days of age. In each image a few myelinated axons are apparent. Five re-imaged layer 6 neurons are numbered. The yellow box surrounds the same set of boutons imaged in each session. Note that by the final session the image quality had degraded slightly, so some fine detail was lost. Scale bar: 50 microns.