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. Author manuscript; available in PMC: 2014 Aug 18.

Published in final edited form as: J Clin Immunol. 2013 Mar 20;33(5):991–1001. doi: 10.1007/s10875-013-9882-5

(a) MDM obtained from patients were stimulated with mycobacteria (myc = M. avium, MOI 5) and IFN-α (1,000 IU/ml) added simultaneously to the wells. Supernatant levels (pg/ml) of TNF-α and IL-1β were assayed 20 h after culture stimulation; (b) Gene expression was evaluated in MDM 3 h after stimulation; (c) Alveolar macrophages (AM) obtained from normal donors were plated, stimulated and assayed as above. Results are mean ± SD of three independent experiments. *p<0.05 when compared to cells stimulated with mycobacteria alone.

(a) MDM obtained from patients were stimulated with mycobacteria (myc = M. avium, MOI 5) and IFN-α (1,000 IU/ml) added simultaneously to the wells. Supernatant levels (pg/ml) of TNF-α and IL-1β were assayed 20 h after culture stimulation; (b) Gene expression was evaluated in MDM 3 h after stimulation; (c) Alveolar macrophages (AM) obtained from normal donors were plated, stimulated and assayed as above. Results are mean ± SD of three independent experiments. *p<0.05 when compared to cells stimulated with mycobacteria alone.