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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Cell Rep. 2014 May 15;7(5):1577–1588. doi: 10.1016/j.celrep.2014.04.027

Figure 2. AKAP79/150 anchoring regulates CaN and PKA localization to dendritic spines.

Figure 2

(A) AKAP79 WT, ΔPIX and ΔPKA-mCh localization in maximum intensity projection images of rat hippocampal neurons. (B) Quantification of AKAP79-mCh WT and mutant dendritic spine localization as a spine/dendrite shaft fluorescence intensity ratio. (C) AKAP79ΔPKA causes a loss of PKA-RIIα–CFP from spines relative to 79WT and 79ΔPIX. (D) Quantification of PKA-RIIα-CFP spine/shaft ratios in neurons expressing WT or mutant AKAPs. (E) AKAP79ΔPIX reduces spine localization of CaNA-YFP relative to WT and 79ΔPKA. (F) Quantification of CaNAα-YFP spine/shaft ratios in neurons expressing WT or mutant AKAPs. (G) Merged images of AKAP79-mCh, PKA-RIIα-CFP, and CaNAα-YFP in WT and mutant AKAP expressing neurons. Arrowheads indicate representative spines. Data expressed as mean ± SEM (*p<0.05, **p<0.01 by ANOVA with Dunnett’s post-hoc test; n = 18–23). Scale bars = 10μm