| Reagent | Company (Preferred v. Alternate) | Catalog Number (Preferred v. Alternate) | Quantity per Culture |
| γ-irradiated poly-L-lysine | Sigma-Aldrich (MP Biomedicals) | P5899 (ICN19454405) | 5 mg |
| DMEM (high glucose) | Sigma-Aldrich (cellgro) | MT-50-003-PB (D7777) | 2 L |
| Laminin | BD Biosciences (Sigma-Aldrich) | CB-40232 (L2020) | 1 mg |
| Sodium Bicarbonate (NaHCO3) | Fisher Scientific (Sigma-Aldrich) | BP328-500 (S5761) | 1.51 g |
| HEPES (C8H18N2O4S) | Fisher Scientific (Sigma-Aldrich) | BP310-1 (H6147) | 9.53 g |
| Antibiotic/Antimycotic | Thermo Scientific (Sigma-Aldrich) | SV3007901 (A5955) | 17-20 ml |
| Gentamicin Sulfate | Lonza (Sigma-Aldrich) | BW17-519Z (G1397) | 2-3 ml |
| Donor Equine Sera | Thermo Scientific (Sigma-Aldrich) | SH3007403 (H1270) | 75 ml |
| Fetal Bovine Sera | Thermo Scientific (Sigma-Aldrich) | SH3007103 (F2442) | 25 ml |
| Collagenase (Type IV) | Worthington (Sigma-Aldrich) | LS004189 (C9891) | 0.44 g |
| Trypsin (from Pancreas) | MP Biomedicals (Sigma-Aldrich) | ICN15357125 (T5266) | 1 g |
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