Reagent	Isolation	Wash	Dissociation	Complete
Base Medium	419.25 ml	395.40 ml	297.00 ml	178.00 ml
PSF*	5.00 ml	4.00 ml	3.00 ml	2.00 ml
Gentamicin Sulfate**	0.75 ml	0.60 ml	-	-
Donor Equine Serum	75.00 ml	-	-	-
Fetal Bovine Serum***	-	-	-	20.00 ml
* PSF: penicillin/streptomycin/fungizone cocktail (100x); ** 50 mg/ml concentration; *** Characterized