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. Author manuscript; available in PMC: 2014 Aug 18.
Published in final edited form as: Mech Dev. 2010 Aug 31;127(0):485–495. doi: 10.1016/j.mod.2010.08.004

Figure 2. p-Smad2 immunostaining at later tailbud stages.

Figure 2

(A) Tailbud stage embryos were treated with 100 μM SB-431542 or 0.2% DMSO for 22 hours prior to harvesting at stage 29 or 36. Embryos were stained with anti-p-Smad2 and cleared. (B) Stage 34 embryos stained for p-Smad2 were cleared to reveal specific structures in the head (top) and tail (bottom). Note that some bilateral structures can be seen twice in cleared heads. br:brain, e:eye, ov:otic vesicle, ba:branchial arches, m:mouth, tb:tailbud, f:fin, nt:posterior neural tube, pw:posterior wall of tailbud, cnh:chordoneural hinge.