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. Author manuscript; available in PMC: 2014 Aug 18.
Published in final edited form as: Methods Enzymol. 2012;504:371–391. doi: 10.1016/B978-0-12-391857-4.00019-7

Figure 19.5.

Figure 19.5

Cells expressing different ratios of Cerulean-B Zip and Venus-B Zip were subjected to FLIM–FRET, and the data were analyzed as described in the -Section 3.2. (A) Single cell measurements for a cell expressing the Cerulean-B Zip protein alone (unquenched donor, top right panel; the calibration bar is 10 μm) or a cell co-expressing the Cerulean- and Venus-B Zip proteins (lower right panel). The lifetime distribution for all pixels in each image is displayed on the common polar plot. (B) The average FRET efficiency (left axis) and donor fluorescence lifetime (right axis) were determined for the cell nucleus for 20 cells separate cells, and the results are plotted as a function of the estimated acceptor-to-donor ratio (IA/ID) for each cell.