. Author manuscript; available in PMC: 2014 Aug 18.

Published in final edited form as: J Pain Relief. 2014 Feb 19;Suppl 3:003. doi: 10.4172/2167-0846.S3-003

Table 1.

Primary monoclonal antibodies (mAb) used for flow cytometry.

	Primary mAb	Source	Clone	Isotype	Stock concentration	Working dilution
Surface staining^a	APC-anti-mouse CD45	eBioscience	30-F11	Rat IgG2b	0.2 mg/ml	1:50
	FITC-anti-mouse CD4	BD Biosciences	GK1.5	Rat IgG2b	0.5 mg/ml	1:50
	PerCP-anti-mouse CD3	BD Biosciences	145-2C11	Armenian Hamster IgG1	0.2 mg/ml	1:50
Intracellular staining^b	PE-anti-mouse T-bet	eBioscience	eBio4B10	Mouse IgG1	0.2 mg/ml	1:100
	PE-anti-mouse GATA-3	eBioscience	TWAJ	Rat IgG2b	3 μg/ml	1:100
	PE-anti-mouse IFN-γ	eBioscience	XMG1.2	Rat IgG1	0.2 mg/ml	1:400
	PE-anti-mouse IL-4	eBioscience	11B11	Rat IgG1	0.2 mg/ml	1:100
	PE-anti-mouse TNF-α	eBioscience	MP6-XT22	Rat IgG1	0.2 mg/ml	1:100
	PE-anti-mouse GM-CSF	eBioscience	MP1-22E9	Rat IgG2a	0.2 mg/ml	1:200

For each sample tube, all three mAbs for surface staining were added at the same time.

For each sample tube, one of these mAbs was used during intracellular staining.