Figure 2. Activation of UAS-dTrpA1 in different GAL4 lines is sufficient to activate discrete cleaning movements in the absence of dust.
Cleaning movement activation phenotypes driven by 12 GAL4 lines expressing UAS-dTrpA1. Flies (including controls) were warmed to 30°C to activate the targeted neurons while their cleaning movements were recorded and manually scored (n = 10 flies/GAL4 line; 130 flies total). Ethograms of the scored behaviors are displayed by compressing all mutually exclusive events to a single line per fly. Colors below the movement names correspond to those on the ethograms. White space in each ethogram represents the time flies spent walking or standing in place. The GAL4 lines are grouped into four cleaning movement categories: head, abdomen, thorax and/or wings, and legs. The grid displays increases and decreases from control flies in the fraction of time each line spent performing different cleaning movements. Grid heat map represents the p-values for the comparisons of the different GAL4 lines and control flies (Kruskal–Wallis followed by Mann–Whitney U pairwise tests and Bonferroni correction). Note: R71C07 displays significant increases in both abdominal cleaning and leg rubbing. Although this line is shown in the abdominal cleaning category, it could also be included with leg rubbing. Lines labeled in red are used in experiments shown in Figure 3 and Figure 5. See Video 2, Video 3, Video 4, Video 5, Video 6, Video 7, Video 8, and Video 9 for representative videos and more description of the activation phenotypes of these lines.
Figure 2—figure supplement 1. GAL4 lines expressing UAS-dTrpA1 have different activated cleaning phenotypes at high temperature.
Figure 2—figure supplement 2. Anatomy of GAL4 lines used to activate distinct cleaning movements.
