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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: Nature. 2014 Jun 8;511(7509):366–369. doi: 10.1038/nature13378

Figure 1. Function and structure of the TYMV TLS.

Figure 1

a, The TLS (dashed box) at the 3′ end of the gRNA, with the UPD upstream. AARS (red) valylates the TLS, which can interact with the RDRP (yellow) or eEF1A (blue). Ribosome binding (green) was suspected but untested. b, Topology of tRNA and the TLS in rainbow colors. 5′ ends are blue and 3′ ends are red. Attached amino acid is aa or val. T= T-loop, D= D-loop, AC= anticodon loop, V= variable loop. c, Secondary structure of the crystallized RNA. Lowercase letter indicates the single mutation. Numbering is from the 5′ end of the crystallized sequence. d, Three views of the structure, colored to match panel c. The conformation of the 3′ CCA and AC loop differ from tRNA, likely due to crystal packing (Extended Data Fig. 8).