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. Author manuscript; available in PMC: 2015 Aug 14.
Published in final edited form as: Chem Biol. 2014 Aug 14;21(8):967–976. doi: 10.1016/j.chembiol.2014.06.008

Figure 3.

Figure 3

ERdj3 is functionally involved in the degradation of L444P GCase, but not WT GCase. (A) Silencing ERdj3 significantly reduced the rate of degradation of L444P GCase in fibroblasts, as assessed by cycloheximide–chase analysis. (B) Quantification of L444P GCase bands when NT (dashed line) and ERdj3 (solid line) siRNA were applied. (C) Silencing ERdj3 did not change the rate of degradation of WT GCase in fibroblasts, as assessed by cycloheximide–chase analysis. The data in (B) are reported as mean ± SEM (n = 4). CHX, cycloheximide. See also Figure S4.