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. 2014 Jul 28;111(32):E3316–E3324. doi: 10.1073/pnas.1406123111

Fig. 4.

Fig. 4.

Mutant β-catenin is required for survival of MYC lymphomas through regulation of apoptotic factors. (A) MYC lymphoma cells expressing DD-ICAT were treated with TMP and levels of apoptosis were tracked by staining for Annexin-V-PE and 7AAD. (B) Western blot analysis for apoptotic proteins in cells treated with TMP for 0, 1, 2, and 3 d. (C) Levels of proapoptotic Bim and antiapoptotic Bcl-xL mRNA in cells treated with TMP for 0, 1, 2, and 3 d, as determined by qRT-PCR. (D) Quantification of apoptosis as measured by staining with Annexin-V after treatment with TMP in cells expressing Bim shRNA or Bcl-xL cDNA compared with empty vector control. All values represent means of at least three independent experiments ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. (E) MYC lymphoma cells expressing DD-ICAT were either untreated, treated with TMP (1 μM), or treated with DOX for 24 h. Cells were fixed and stained with PI to measure DNA content. Histogram events are gated on viable cells.