Fig. 1.

Analysis of yeast deletion mutants, which showed increased sensitivity to Tcin. (A) The mutants were classified based on MIPS FunCat Database terms such as signaling and protein modification (SP), ribosome biogenesis and protein degradation (Protein), RNA degradation and stability (RNA), DNA repair and damage response (DNA), VPS, mitochondria (Mito), unclassified (UC), cell cycle (CC), and the remaining characterized mutants were grouped as Other. (B) The top 27 mutants exhibiting the highest sensitivity to Tcin and BY4743 were grown with or without 1 µM Tcin and in the presence or absence of 4.5 mM vitamin E. At 18 h after treatment, growth at OD₆₀₀ was measured and relative growth was calculated as the ratio of growth of treated cells to that of untreated cells, with a ratio of 1 indicating no effect on growth. (C) Following 1 h after treatment of the Tcin-sensitive strains and BY4743 with 1 µM Tcin, equal OD₆₀₀ cells were stained with DCFH-DA. The ratio of ROS-positive cells for each Tcin-treated mutant was normalized to the ratio of ROS-positive cells for Tcin-treated BY4743, and fold increase was plotted. Error bars represent SE calculated from four independent experiments. Differences relative to BY4743 were assessed by using ANOVA followed by post hoc LSD tests (*P < 0.05).