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Incorporation of T opposite 7-EAA and 7-EAA triazole by AMV-RT reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing different ddNTPs and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.
