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. 2014 Jun 5;9(8):1788–1798. doi: 10.1021/cb500086e

Figure 3.

Figure 3

Phytochemicals inhibit mechanosensitive channels. MscL channels were reconstituted into calcein-loaded vesicles. Channel activation was initiated by exposure to MTSET and the release of calcein through open MscL channels is monitored as an increase in fluorescence. (a) Representative calcein release curves. (b, c) Changes in MscL activity after addition of phytochemicals, avg ± standard deviation (SD), n = 3. The solid lines are f([mod]) = f(control) – [mod]/D fits to the results (excluding saturating concentration). (b) The phytochemicals produce a 50% reduction in the max release at (concentrations in μM) 127 ± 5 capsaicin, 2.7 ± 0.2 curcumin, 28 ± 2 EGCG, 82 ± 2 genistein, and 63 ± 2 resveratrol μM concentration and half the efflux rate (c) at 92 ± 12 capsaicin, 1.6 ± 0.4 curcumin, 18 ± 5 EGCG, 46 ± 9 genistein, and 40 ± 9 resveratrol.