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. 2014 Jun 20;9(8):1877–1884. doi: 10.1021/cb5004114

Figure 2.

Figure 2

SPR responses for the interaction of HA protein (0.4 μM) with glycolipids on an SLB array. In each spot (curve), the concentration of α2-3 linked sialic acid 33-ϕ-OEG-lipid is kept constant (0.8 mol %), while the concentration of the second lipid molecule (A, ϕ-OEG-lipid; B, H2N-OEG-lipid) is varied from 0% to 0.05, 0.1, 0.3, 0.5, 0.8, 1.0, 1.5, 2.0, 3.0, and 4.0 mol %. In panel A, the concentrations of ϕ-OEG-lipid (0–4%) are represented by gray scale with increasing darkness. The red curves are fits to eq 3 and the green curves to eq 6. In panel B, the 0% H2N-OEG-lipid concentration is represented by the blue curve, with all other concentrations as gray curves.