Table 1. Kinetic Parameters for HA Binding to 33-ϕ-OEG-Lipid in the Supported Lipid Bilayer with Different Concentrations of ϕ-OEG-Lipida.
| ϕ-OEG-lipid (%) | α (nm–2) | β (s–1) | βd = kd (s–1) | ka (s–1 M–1) | KD (μM) | [PGx]0 (nm–2) | [PG]0 (nm–2) |
|---|---|---|---|---|---|---|---|
| 0 | 1.3 × 10–3 | 4.0 × 10–3 | 1.0 × 10–2 | 1.0 × 104 | 1.0 | 7.8 × 10–4 | 2.0 × 10–4 |
| 0.05 | 2.0 × 10–3 | 3.4 × 10–3 | 7.3 × 10–3 | 8.5 × 103 | 0.86 | 1.1 × 10–3 | 3.0 × 10–4 |
| 0.1 | 2.2 × 10–3 | 3.6 × 10–3 | 7.4 × 10–3 | 9.0 × 103 | 0.82 | 1.1 × 10–3 | 3.5 × 10–4 |
| 0.3 | 2.6 × 10–3 | 3.1 × 10–3 | 7.2 × 10–3 | 7.8 × 103 | 0.94 | 1.3 × 10–3 | 3.3 × 10–4 |
| 0.5 | 3.8 × 10–3 | 2.5 × 10–3 | 7.3 × 10–3 | 6.3 × 103 | 1.4 | 1.6 × 10–3 | 3.9 × 10–4 |
| 0.8 | 3.6 × 10–3 | 2.9 × 10–3 | 7.2 × 10–3 | 7.2 × 103 | 1.0 | 1.9 × 10–3 | 3.8 × 10–4 |
| 1 | 5.0 × 10–3 | 2.2 × 10–3 | 7.5 × 10–3 | 5.5 × 103 | 1.4 | 2.0 × 10–3 | 4.0 × 10–4 |
| 1.5 | 5.0 × 10–3 | 2.3 × 10–3 | 7.3 × 10–3 | 5.7 × 103 | 1.3 | 2.2 × 10–3 | 3.9 × 10–4 |
| 2 | 6.6 × 10–3 | 1.8 × 10–3 | 8.1 × 10–3 | 4.4 × 103 | 1.8 | 2.3 × 10–3 | 3.9 × 10–4 |
| 3 | 8.0 × 10–3 | 1.5 × 10–3 | 7.9 × 10–3 | 3.7 × 103 | 2.1 | 2.5 × 10–3 | 4.0 × 10–4 |
| 4 | 8.0 × 10–3 | 1.6 × 10–3 | 8.0 × 10–3 | 4.0 × 103 | 2.0 | 2.6 × 10–3 | 3.9 × 10–4 |
a
The estimated standard deviation is within ±10% for each parameter.