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L1 retrotransposition in ATM+/– cells
| Cell line | ATM | Test | No. L1 coloniesa (±SD) | No. neoR plasmid coloniesb (±SD) |
|---|---|---|---|---|
| YZ-5 | + | 1 | 62 (6) | 207 (38) |
| EBS7 | – | 1 | 0 | 133 (10) |
| YZ-5 | + | 2 | 24 (10) | 115 (12) |
| EBS7 | – | 2 | 0 | 91 (4.7) |
Optimized human L1 with selection cassette synwt-opt ORF1-neo.
Neomycin-resistance expression plasmid pIRES-EGFP.
