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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Clin Cancer Res. 2014 Jan 14;20(6):1566–1575. doi: 10.1158/1078-0432.CCR-13-2195

Figure 5. GANT61 is more efficient than GDC-0449 in suppressing the expression of GLI target genes.

Figure 5

A∼B. Real-time PCR of GAPDH-normalized mRNA level of PTCH1 (A) and GLI2 (B) in cells treated with DMSO or 10 μmol/L GDC-0449 for 96 hours. C∼E. 96-hour exposure to 10 μmol/L GANT61 reduced mRNA levels of GLI targets. Gene expressions were normalized to endogenous GAPDH in each cell line. Data represent the mean ± SD of 3 independent experiments. (Two-tailed t test, *: p<0.05; **: p<0.01; ***: p<0.001) F. 96-hour treatment of 10 μmol/L GANT61 reduced protein levels of GLI2 and CCND1, and induced cleaved caspase-3 and cleaved PARP. GAPDH was used as the loading control. A representative Western blot from 3 independent experiments is shown.