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. Author manuscript; available in PMC: 2015 Aug 17.
Published in final edited form as: Toxicol Lett. 2014 Jun 6;229(1):198–209. doi: 10.1016/j.toxlet.2014.06.009

Figure 5. CNT are potent suppressors of mast cell calcium fluxes.

Figure 5

A. Left panel. RBL2H3 cells were pre-incubated with 10μg/ml SWCNT for the indicated times (in days) prior to IgE loading and preparation for calcium assay. Fluo-4 loaded cells were stimulated with 200ng/ml KHL-DNP (black bar) after establishing a baseline (grey bar). Right panel. Area Under the Curve (AUC) analysis of calcium responses in cells pre-incubated with 100μg/ml SWCNT. AUC for 0-120s were integrated (Prism) from cells stimulated with either IgE/KLH-DNP or ionomycin after the 2-180 min pre-incubation times indicated. B. Dose response analysis of SWCNT effects upon ionomycin and antigen (KLH-DNP)-induced calcium responses in RBL2H3 cells. AUC of 120s calcium traces were calculated for cells stimulated as indicated with 1h pre-incubation of the indicated SWCNT doses. C. Comparison AUC analysis of suppression of antigen (KLH-DNP) and ionomycin induced calcium responses 0-180 min pre-incubation with 100μg/ml SWCNT. D, E. Matched calcium assay of control cells (D) and those pre-stimulated with 100μg/ml SWCNT for 1h (E). Indicated stimuli were added at the 20s timepoint. Experiment was performed in the presence of external calcium. F. Matched calcium assay of control cells and those pre-stimulated with 100μg/ml SWCNT for 1h. Indicated stimuli were added at the 20s timepoint. Experiment was performed in nominally calcium-free external medium. G. Growth curve of RBL2H3 incubated in the absence or presence of 100μg/ml SWCNT for the indicated time (in days).