Table 1. Coverage and read depth following targeted capture and next generation sequencing for the 4 patient verification study.

Sample	Tag	Aligned Reads	Reads on target	% Reads on target	Mean coverage	% ≥5	% ≥10	% ≥15	% ≥20*	% ≥30	% ≥50
Patient A tag1	CAACCT	6,296,720	1,310,107	20.8%	133	98.5	97.7	96.8	95.7	93.3	86.5
Patient B tag2	AACCAT	5,788,340	1,359,182	23.5%	137	98.4	97.5	96.6	95.6	93.4	87.9
Patient C tag3	AAGGAT	8,539,613	2,174,816	25.5%	220	98.7	98.2	97.6	96.9	95.9	93.1
Patient D tag4	AATTAT	4,314,207	1,609,443	37.3%	164	98.5	97.6	96.7	95.8	93.9	88.8
Patient tag1 to 4, AVERAGE						98.5	97.8	96.9	96.0	94.1	89.1

The tagging, aligned reads, reads on target, % reads on target, mean coverage and % coverage with a particular minimum read depth are shown for each patient DNA. The asterix highlights the % coverage with a greater than or equal to 20 read depth.