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. 2014 Aug 18;9(8):e105223. doi: 10.1371/journal.pone.0105223

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© 2014 Zorin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The pRbcS450 plasmid (A) contains the selection cassette, flanked by NotI restriction sites. The pJET-LiD5Des plasmid (B) contains the functional DES5 gene with its flanking regions. Following NotI digestion, the selection cassette was subcloned into pJET-LiD5Des. Primers used in analysis of P127-transformant are marked.